March 1998
Volume 39, Issue 3
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Articles  |   March 1998
Indirect mitogenic effect of transforming growth factor-beta on cell proliferation of subconjunctival fibroblasts.
Author Affiliations
  • E P Kay
    Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.
  • H K Lee
    Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.
  • K S Park
    Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.
  • S C Lee
    Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.
Investigative Ophthalmology & Visual Science March 1998, Vol.39, 481-486. doi:
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    • Get Citation

      E P Kay, H K Lee, K S Park, S C Lee; Indirect mitogenic effect of transforming growth factor-beta on cell proliferation of subconjunctival fibroblasts.. Invest. Ophthalmol. Vis. Sci. 1998;39(3):481-486.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To understand the mechanism of fibrosis after filtering surgery for glaucoma, the effect of transforming growth factor-beta (TGF-beta) was studied in subconjunctival fibroblasts (SCFs). TGF-beta, universal inhibitor of cell proliferation, stimulates the cell proliferation of fibroblasts. SCFs were evaluated for their production of TGF-beta and fibroblast growth factor 2 (FGF-2) to determine whether TGF-beta may be an indirect mitogen acting through the induction of an endogenous growth factor, or factors, that then acts as the direct mitogen in an autocrine manner. METHODS: Cell proliferation was determined either by counting cell numbers or by analyzing the incorporation of [3H]thymidine into DNA. The synthesis of TGF-beta and FGF-2 was analyzed by immunoprecipitation and immunoblotting. RESULTS: TGF-beta 1, TGF-beta 2, and TGF-beta 3 stimulated the cell proliferation of SCFs in a dose-dependent manner. The media conditioned by SCFs, which were subsequently activated by acid, stimulated cell proliferation of corneal stromal fibroblasts. When the acid-activated media conditioned by SCFs were immunoprecipitated, respectively, either with anti-TGF-beta 1 and TGF-beta 2 antibodies or with anti-TGF-beta 3 antibody, TGF-beta s, with an apparent molecular size of 25 kDa, were detected, whereas SCFs produced an 80-kDa latent form of TGF-beta 1. Interestingly, SCFs produced and secreted an 18-kDa extracellular isoform of FGF-2, the synthesis of which is further stimulated by TGF-beta 1 and TGF-beta 3, respectively, whereas the neutralizing antibody to FGF-2 and the FGF-2-specific antisense oligonucleotide primers inhibited the stimulatory activities of TGF-beta 1 in SCFs. CONCLUSIONS: These findings indicate that SCFs produce TGF-beta and FGF-2 and that FGF-2 seems to be the direct stimulator of TGF-beta-mediated cell proliferation in SCFs.

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