June 1999
Volume 40, Issue 7
Free
Articles  |   June 1999
Effects of UV-A radiation on lens epithelial NaK-ATPase in organ culture.
Author Affiliations
  • A Dovrat
    B. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
  • O Weinreb
    B. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
Investigative Ophthalmology & Visual Science June 1999, Vol.40, 1616-1620. doi:
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      A Dovrat, O Weinreb; Effects of UV-A radiation on lens epithelial NaK-ATPase in organ culture.. Invest. Ophthalmol. Vis. Sci. 1999;40(7):1616-1620.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To investigate the mechanisms involved in the damage caused by UV-A irradiation at 365 nm on the eye lens. METHODS: Bovine lenses obtained from animals 1 to 5 years of age were placed in specially designed organ culture chambers for preincubation. Twenty-four hours later, the lenses were irradiated by 33 J/cm2 UV-A at 365 nm. During irradiation, the lenses were oriented in the culture so that the anterior surface faced the incident UV-A radiation source. After irradiation, lens optical quality was monitored throughout the 8 days of the culture period, and lens samples were taken for analysis of NaK-ATPase activity. RESULTS: Lens optics and NaK-ATPase activity were affected by irradiation of 33 J/cm2. The effects on lens epithelial NaK-ATPase activity were stronger at the equators than at the center. The damage to the activity at the center was reversible, as the lens optically recovered from the LW-A damage. CONCLUSIONS: Lens NaK-ATPase activity can recover from damage caused by UV-A at 365 nm. When the lenses received irradiation of 33 J/cm2, NaK-ATPase activity recovered from the damage during the culture period only at the center and not at the equators of the epithelium.

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