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Xin-Mei Zhang, Bai-Yu Chen, Alam Hoi-Lam Ng, Julian A. Tanner, David Tay, Kwok-Fai So, Rivka A. Rachel, Neal G. Copeland, Nancy A. Jenkins, Jian-Dong Huang; Transgenic Mice Expressing Cre-Recombinase Specifically in Retinal Rod Bipolar Neurons. Invest. Ophthalmol. Vis. Sci. 2005;46(10):3515-3520. doi: 10.1167/iovs.04-1201.
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purpose. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants.
methods. The IRES-Cre-cDNA fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity.
results. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina.
conclusions. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation.
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