Purchase this article with an account.
Aya Takeuchi, Yoshihiko Usui, Masaru Takeuchi, Takaaki Hattori, Takeshi Kezuka, Jun Suzuki, Yoko Okunuki, Takuya Iwasaki, Makoto Haino, Kouji Matsushima, Masahiko Usui; CCR5-Deficient Mice Develop Experimental Autoimmune Uveoretinitis in the Context of a Deviant Effector Response. Invest. Ophthalmol. Vis. Sci. 2005;46(10):3753-3760. doi: 10.1167/iovs.04-1429.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. Experimental autoimmune uveoretinitis (EAU) is an organ-specific, Th1-cell–mediated disease that targets the neural retina. CCR5 is a chemokine receptor expressed on Th1 cells that promotes their migration. In CCR5-deficient mice, we examined the role of CCR5 in the development of EAU induced by immunization with interphotoreceptor retinoid-binding protein (IRBP) peptide.
methods. Wild-type or CCR5-deficient B6 mice were immunized with human IRBP peptide 1-20 (hIRBP-p), and the severity of EAU was assessed clinically and histologically. Splenocytes and cells of regional lymph nodes near the eye were collected and their proliferation and production of IL-6, IL-10, IFN-γ, and CCL2 (MCP-1) in response to hIRBP-p stimulation were measured. Moreover, the intraocular levels of these cytokines were analyzed.
results. Immunization with hIRBP-p induced EAU in CCR5-deficient mice with a severity comparable to that in wild-type mice. Histologically, T-cell infiltration of the eye was reduced, but granulocyte infiltration was augmented in CCR5-deficient mice. Although splenic T cells from CCR5-deficient mice produced IFN-γ but not IL-10 on stimulation by hIRBP-p, T cells from the regional lymph nodes failed to produce both cytokines. IL-6 production in the eye and IL-6 and CCL2 production by splenic T cells were predominantly augmented in CCR5-deficient mice.
conclusions. The development of EAU is not prevented in CCR5-deficient mice. Although T-cell infiltration into the eye is apparently reduced in CCR5-deficient mice, the defect is compensated for by granulocyte infiltration, supposedly mediated by augmented intraocular production of IL-6.
This PDF is available to Subscribers Only