Eagle’s minimum essential medium (MEM), fetal bovine serum, and trypsin-EDTA (0.5% and 5.3 mM [tetrasodium salt], respectively] were obtained from Invitrogen-Gibco (Rockville, MD); acid-solubilized type I collagen from porcine tendon (Cellmatrix type I-A; 3 mg/mL; native form) and type IV collagen from bovine anterior lens capsule (Cellmatrix type IV; 3 mg/mL; prepared by pepsin treatment), as well as reconstitution buffer (50 mM NaOH, 260 mM NaHCO3, 200 mM HEPES [pH 7.3]) were from Nitta Gelatin (Yao, Osaka, Japan). Chondroitin sulfate A (sodium salt; whale cartilage), chondroitin sulfate C (sodium salt; shark cartilage), dermatan sulfate (sodium salt; hog skin), heparan sulfate (sodium salt; bovine kidney), keratan sulfate (sodium salt; bovine cornea), and hyaluronic acid (sodium salt; hog skin) were from Seikagaku (Tokyo, Japan). Dulbecco’s phosphate-buffered saline (PBS) was from Nissui Pharmaceutical (Tokyo, Japan), bovine serum albumin (BSA; fraction V) was from Nacalai Tesque (Kyoto, Japan), fibronectin (human plasma) was from Roche Molecular Biochemicals (Mannheim, Germany), and laminin (1 mg/mL; purified from Engelbreth-Holm-Swarm [EHS] mouse tumor basement membrane, classic laminin) was from Upstate Biotechnology (Lake Placid, NY). Rabbit antiserum to integrin α5 and mouse monoclonal antibodies to integrin β1 were from Chemicon (Temecula, CA). Cytochalasin D, nocodazole, paraformaldehyde, Triton X-100, and Tween 20 were from Sigma-Aldrich (St. Louis, MO). The peptides GRGDSP and GRGESP were from American Peptide (Sunnyvale, CA). Cell culture clusters (24-well) and 162-cm2 cell culture flasks were from Corning (Corning, NY), and glass-bottomed dishes were from Matsunami Glass (Kishiwada, Osaka, Japan). Fluorophore-labeled phalloidin (Alexa Fluor 568) and goat antibodies to mouse or rabbit IgG (Alexa Fluor 488) were from Molecular Probes (Eugene, OR). Normal mouse IgG was from Santa Cruz Biotechnology (Santa Cruz, CA), and normal rabbit serum was from Cappel (Durham, NC).