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Noemi Lois, Julie Taylor, Alastair D. McKinnon, Gillian C. Smith, Rob van’t Hof, John V. Forrester; Effect of TGF-β2 and Anti–TGF-β2 Antibody in a New In Vivo Rodent Model of Posterior Capsule Opacification. Invest. Ophthalmol. Vis. Sci. 2005;46(11):4260-4266. doi: 10.1167/iovs.05-0168.
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purpose. This study evaluated the effect of transforming growth factor (TGF)-β2 and anti–TGF-β2 antibody in a rodent model of posterior capsule opacification (PCO).
methods. An extracapsular lens extraction (ECLE) was performed in 72 Sprague–Dawley rats. At the end of the procedure, 10 μL TGF-β2 (TGF-β2–treated group), fetal calf serum (FCS)/phosphate-buffered saline (PBS; FCS/PBS-treated control group), a human monoclonal TGF-β2 antibody (anti–TGF-β2–treated group), or a null control IgG4 antibody (null antibody–treated control group) was injected into the capsule. Animals were killed 3 and 14 days postoperatively. Eyes were evaluated clinically prior to euthanatization, then enucleated and processed for light microscopy and immunohistochemistry afterward. PCO was evaluated clinically and histopathologically. Student’s t-test and χ2 were used to assess differences between groups.
results. There were no statistically significant clinical or histopathological differences in degree of PCO between the TGF-β2– and FCS/PBS-treated groups at 3 and 14 days after ECLE. Nor were there differences between the anti–TGF-β2– and the null antibody–treated groups, with the exception of the histopathology score for capsule wrinkling 3 days after ECLE (P = 0.02). α-Smooth-muscle actin staining was observed in the lens capsular bag only in areas where there was close contact with the iris.
conclusions. No sustained effect of TGF-β2 or anti–TGF-β2 antibody on PCO was found in rodents at the dose and timing administered in this study. Iris cells may play a role in the process of epithelial mesenchymal transition linked to PCO.
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