Onset of GFP expression occurred within 3 days of injection of 5 × 10
8 vp scAAV.GFP and within 7 days of injection of 5 × 10
8 vp ssAAV.GFP (
Fig. 2 , top panel). Although the onset of expression was later for ssAAV.GFP, by 28 days both showed transduction of ganglion cells and some cells in the inner nuclear layer throughout the entire retina. Compared with the highest dose, onset of expression was delayed after the injection of 5 × 10
7 vp scAAV.GFP, but there was still expression of GFP in ganglion cells and some cells in the inner nuclear layer throughout the retina, whereas GFP expression was limited to ganglion cells after the injection of 5 × 10
7 vp ssAAV.GFP (
Fig. 2 , middle panel). After the injection of 10
7 vp, GFP intensity was reduced compared with that in higher doses of scAAV.GFP, but expression was still widespread in ganglion cells and some cells in the inner nuclear layer throughout the retina (
Fig. 2 , lower panel). In contrast, after injection of10
7 vp ssAAV.GFP, no GFP expression was detectable before 28 days, and then it was limited to faint expression in ganglion cells.