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Kenji Sugisaki, Tomohiko Usui, Nobuhiro Nishiyama, Woo-Dong Jang, Yasuo Yanagi, Satoru Yamagami, Shiro Amano, Kazunori Kataoka; Photodynamic Therapy for Corneal Neovascularization Using Polymeric Micelles Encapsulating Dendrimer Porphyrins. Invest. Ophthalmol. Vis. Sci. 2008;49(3):894-899. doi: 10.1167/iovs.07-0389.
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purpose. To investigate the accumulation of new photosensitizers (PSs), dendrimer porphyrin (DP, free DP), and DP encapsulation into polymeric micelles (DP-micelle) and the efficacy of photodynamic therapy (PDT) in an experimental corneal neovascularization model in mice.
methods. Corneal neovascularization was induced by suturing 10–0 nylon 1 mm away from the limbal vessel in C57BL6/J mice. To determine the accumulation of free DP and DP-micelle, 10 mg/kg free DP or DP-micelle was administered by intravenous injection 4 days after suture placement. Mice were killed 1, 4, 24, and 168 hours after the injection of PS. Twenty-four hours after the administration of free DP or DP-micelle, mice were treated with a diode laser of 438-nm wavelength at 10 or 50 J/cm2. Fluorescein angiography was performed before and 7 days after irradiation, and the area of corneal neovascularization was quantified.
results. Free DP and DP-micelle accumulated in the neovascularized area 1 hour to 24 hours after administration. Fluorescence of DP was weaker than that of DP-micelle. Neither DP-micelle nor DP could be detected in normal limbal vasculature. In the PDT experiments using PS, mean residual rates of corneal neovascularization were 10.1% in the mice treated with DP-micelle and 21.6% in the mice treated with free DP at 10 J/cm2 (P < 0.01). At 50 J/cm2, mean residual rates of corneal neovascularization were 10.6% in the mice treated with DP-micelle and 13.7% in the mice treated with free DP (P > 0.05). Although corneal neovascularization in PDT-treated mice exhibited significant regression compared with the control group, significant energy-related vessel regression with increasing laser energy could not be observed.
conclusions. PDT with DP-micelle and free DP can provide efficacious treatment of corneal neovascularization.
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