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Shengxiu Li, Dan Goldowitz, Douglas J. Swanson; The Requirement of Pax6 for Postnatal Eye Development: Evidence from Experimental Mouse Chimeras. Invest. Ophthalmol. Vis. Sci. 2007;48(7):3292-3300. doi: 10.1167/iovs.06-1482.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. The small eye mouse mutant (Sey) is caused by a mutation of the Pax6 gene. Previous studies, in which aggregation chimeras were used, have demonstrated that Sey/Sey cells contribute poorly to the neural retina forming small clumps of cells restricted to the inner retina at embryonic day 16.5. In addition, Sey/+ cells are absent from the lens epithelium during this embryonic period and postnatally. This study was conducted to determine the fates of these Sey/Sey and Sey/+ cells with continued development in chimeric mouse eyes.
methods. Observations were made on heterozygous and homozygous Sey cells in chimeric eyes from postnatal day (P)0 to P10.
results. In Sey/Sey↔wild-type (wt) chimeras, all Sey/Sey cells originating from retinal progenitor cells died at perinatal times. The only remaining Sey/Sey cells in the neural retina were associated with blood vessels, including vascular endothelial cells, pericytes, astrocytes, and microglia, which have extraretinal origins. In contrast, Sey/+ cells formed all retinal cell classes. As previously reported, Sey/Sey cells were absent from the lens and corneal epithelium. However, in contrast to previous reports, Sey/+ cells contributed to the lens epithelium as well as corneal tissues, and Sey/Sey cells were absent from the anterior retinal pigment epithelium.
conclusions. All evidence showed that, when Pax6 is absent at the initial stages of the development, Sey/Sey cells that contribute to the neural retina die, even when wild-type cells are available to provide normal environmental cues.
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