The CCTs of WT, heterozygous, and SPARC-null mice were measured by OCT, UBM, and histology for the same groups of mice (
Figs. 3 4 ,
Table 2 ). Average CCTs measured by OCT were 105.6 ± 4.3, 109.1 ± 3.6, and 104.5 ± 3.9 μm for WT, heterozygous, and SPARC-null mice, respectively (
n = 14, 12, and 6, respectively). The CCTs of WT, heterozygous, and SPARC-null mice by OCT were significantly different by ANOVA (
P < 0.05) because CCTs of heterozygous mice were trending toward being significantly larger than WT and SPARC-null CCTs (
P = 0.104, 0.09, respectively). Average CCTs measured by UBM were 110.7 ± 3.9, 114.9 ± 5.1, and 106.9 ± 7.1 μm for WT, heterozygous, and SPARC-null mice, respectively (
n = 14, 12, and 6, respectively). The CCTs of WT, heterozygous and SPARC-null mice by UBM were significantly different by ANOVA (
P < 0.01) with the CCTs of heterozygous mice significantly larger than those of SPARC-null (
P < 0.01). Average CCTs measured by histology were 74.0 ± 8.6, 78.5 ± 12.5, and 74.3 ± 6.3 μm for WT, heterozygous, and SPARC-null mice, respectively (
n = 14, 12, and 6, respectively). The CCTs of the eyes from all three genotypes by histology were not significantly different, by ANOVA (
P = 0.488).