Purchase this article with an account.
Kyle C. McKenna, Kelly M. Beatty, Richard A. Bilonick, Lynn Schoenfield, Kira L. Lathrop, Arun D. Singh; Activated CD11b+ CD15+ Granulocytes Increase in the Blood of Patients with Uveal Melanoma. Invest. Ophthalmol. Vis. Sci. 2009;50(9):4295-4303. doi: 10.1167/iovs.08-3012.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To determine whether activated CD11b+ CD15+ granulocytes increase in the blood of patients with uveal melanoma.
methods. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation from the blood of patients with primary choroidal/ciliochoroidal uveal melanomas (six women, four men; age range, 46–91 years) and healthy control donors (14 women, 10 men; age range, 50–81 years). The expression of CD15 and CD68 on CD11b+ myeloid cells within PBMCs and primary uveal melanomas was evaluated by flow cytometry. CD3ζ chain expression by CD3ε+ T cells in PBMCs and within primary uveal melanomas was measured as an indirect indication of T-cell function.
results. The percentage of CD11b+ cells in PBMCs of patients with uveal melanoma increased 1.8-fold in comparison to healthy donors and comprised three subsets: CD68 negative CD15+ granulocytes, which increased 4.1-fold; CD68− CD15− cells, which increased threefold; and CD68+ CD15low cells, which were unchanged. A significant (2.7-fold) reduction in CD3ζ chain expression on CD3ε+ T cells, a marker of T-cell dysfunction, was observed in PBMCs of patients with uveal melanoma in comparison with healthy control subjects and correlated significantly with the percentage of CD11b+ cells in PBMCs. CD3ζ chain expression on T cells within primary tumors was equivalent to CD3ζ expression in PBMCs of the same patient in four of five patients analyzed.
conclusions. Activated CD11b+ CD15+ granulocytes expand in the blood of patients with uveal melanoma and may contribute to immune evasion by ocular tumors by inhibiting T-cell function via decreasing CD3ζ chain expression.
This PDF is available to Subscribers Only