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Kerstin Steindl-Kuscher, Walter Krugluger, Michael E. Boulton, Paulina Haas, Karl Schrattbauer, Hans Feichtinger, Wolfram Adlassnig, Susanne Binder; Activation of the β-Catenin Signaling Pathway and Its Impact on RPE Cell Cycle. Invest. Ophthalmol. Vis. Sci. 2009;50(9):4471-4476. doi: 10.1167/iovs.08-3139.
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purpose. To investigate the effect of EGF, IGF-1, and VEGF on ARPE19 cell proliferation and differentiation.
methods. The gene expression of RPE-specific differentiation and proliferation markers and the transcriptional and translational activity of β-catenin signaling markers were measured by flow cytometry and RT-PCR.
results. The data showed a significant decrease in RPE65, CRALBP, and cytokeratin 18 in ARPE-19 cells stimulated with EGF and IGF-1. In addition, a significant decrease in GSK-3β and β-catenin was observed that was paralleled by an increase in cyclin D1 expression. Cell cycle studies revealed an increase in ARPE cells in the S-G2/M-phase after treatment with EGF or IGF-1. VEGF, on the other hand, led to a reduction in cyclin D1 and to an increase in GSK 3β and β-catenin expression which was paralleled by an increase in RPE-specific differentiation markers.
conclusions. The data demonstrate the induction of proliferation by EGF and IGF-1 and upregulation of the β-catenin signaling pathway in ARPE-19 cells. The data suggest that activation of the β-catenin signaling pathway may be key in activating ARPE-19 cells by different growth factors.
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