Purchase this article with an account.
Heather M. Cathcart, Mei Zheng, Jason J. Covar, Yi Liu, Robert Podolsky, Sally S. Atherton; Interferon-gamma, Macrophages, and Virus Spread after HSV-1 Injection. Invest. Ophthalmol. Vis. Sci. 2011;52(7):3984-3993. doi: 10.1167/iovs.10-6449.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
After uniocular anterior chamber (AC) injection of HSV-1, the anterior segment of BALB/c mice becomes inflamed and infected; however, virus does not spread from the anterior segment to cause retinitis in the injected eye. The purpose of these studies was to determine whether interferon (IFN-)-γ and Mac-1+ cells play a role in preventing direct anterior-to-posterior spread of HSV-1 in the injected eye.
One AC of adult female BALB/c mice was injected with HSV-1 (KOS). The location of IFN-α, IFN-β, and IFN-γ in the injected eye was determined by immunofluorescence, and mRNA expression was quantified by qPCR. Injected eyes of IFN-γ knockout or clodronate-treated macrophage-depleted mice were examined to determine whether the absence of IFN-γ or Mac-1+ macrophages affected the sites or timing of virus spread.
IFN-α, IFN-β, and IFN-γ were observed in the anterior segment of injected eyes through 72 hours and mRNA levels of IFN-β and IFN-γ were increased in virus-infected eyes 48 to 120 hours after infection. However, the absence of IFN-γ or macrophages did not affect either the sites or the timing of HSV-1 infection in injected eyes.
Protection of the retina of the injected eye does not depend on a single cell type or cytokine. In addition, in the eye, as in other sites of the body, there are redundancies in the innate response to virus infection.
This PDF is available to Subscribers Only