After preliminary fixation, specimens then passed through the following program at 20°C: (1) 0.1 M cacodylate, 5 minutes; (2) 70% ethanol, 10 minutes; (3) 95% ethanol, 10 minutes; (4) 95% ethanol, 10 minutes; (5) 100% ethanol, 10 minutes; (6) 100% ethanol, 10 minutes; (7) 100% ethanol, 10 minutes; (8) propylene oxide, 10 minutes; (9) propylene oxide, 10 minutes; (10) PO:agar resin 2:1, 20 minutes; (11) PO:agar resin 1:1, 40 minutes; (12) PO:agar resin 1:2, 1 hour; (13) agar resin, 1 hour; (14) agar resin, 4 hours; (15) agar resin, 4 hours; and (16) agar resin, 6 hours.