Fifty-eight genes were cloned only from Y79 cells, and the major functional group consisted of 15 transcription factors:
RAX2 (Ne*/Nt* = 1.0, 5 clones),
CRX (Ne*/Nt* = 0.92, 2 clones),
RAX (Ne*/Nt* = 0.90, 1 clone),
NRL (Ne*/Nt* = 0.83, 7 clones),
LHX3 (Ne*/Nt* = 0.73, 9 clones),
OTX2 (Ne*/Nt* = 0.67, 3 clones),
ATOH7 (Ne*/Nt* = 0.60, 4 clones),
VAX2 (Ne*/Nt* = 0.40, 1 clone),
NEUROG1 (Ne*/Nt* = 0.40, 4 clones),
NEUROD1 (Ne*/Nt* = 0.32, 9 clones),
TLX2 (Ne*/Nt* = 0.32, 1 clone),
ISL2 (Ne*/Nt* = 0.32, 1 clone),
HMX1 (Ne*/Nt* = 0.27, 1 clone),
RCOR2 (Ne*/Nt* = 0.23, 2 clones), and
RXRG (Ne*/Nt* = 0.20, 3 clones). The homeodomain-type transcription factors (OTX2, CRX, RAX, RAX2), the bZIP-type transcription factor (NRL), and the bHLH-type transcription factors (ATOH7, NEUROG1, NEUROD1) are known to be involved in the transcription of photoreceptor-specific genes.
9 In addition, LHX3, VAX2, TLX2, ISL2, and HMX1 are classified as homeodomain transcription factors. Finally, RXRG is a negative regulator of S-cones.
48