This study was approved by the Southwest Independent Institutional Review Board and was conducted in accordance with 21 Code of Federal Regulations Parts 50, 54 56, and 812, applicable Bausch & Lomb Standard Operating Procedures, and the Declaration of Helsinki. Thirty healthy volunteers were enrolled in this study and all subjects gave informed consent and were assessed for eligibility. Inclusion criteria were as follows: must be 18 years of age or older and have full legal capacity to volunteer, must have no allergic conjunctivitis, must not be using any topical ocular medications, must have no contact lens wear or use of ophthalmic drops 8 hours before the study visit, must be willing and able to follow instructions, and must have signed a statement of informed consent. Exclusion criteria were: a core project team member, a Research Clinic employee, participation in a conflicting study, or considered by the Investigator not to be a suitable candidate for participation. Subject discontinuation criteria were: adverse effects, other ocular complications, subject noncompliance, subject request, or subject found to be ineligible during study participation. Each subject was clinically evaluated for dry eye, and the order of testing was: OSDI, tear breakup time (TBUT), tear osmolarity, and a 5-minute break was allowed before Schirmer's test.