|MTM cells cultured in a 12-well plate were treated with 0.1% ETH or 100 nM DEX for 10 days. After PBS washing, MTM cells were lysed in 100 μL M-PER lysis buffer (Thermo Scientific). Protein concentration was measured with the Protein DC kit (Bio-Rad Laboratories, Inc.). Total protein (30 μg) was mixed with Laemmli buffer and boiled for 5 minutes before loading. Proteins were separated by 10% SDS-PAGE and then transferred to a polyvinylidine difluoride (PVDF) membrane (Millipore, Billerica, MA). The membrane was blocked with 10% dry milk, and probed with rabbit anti-myocilin antibody (H-130; Santa Cruz Biotechnology, Inc., Santa Cruz, CA) and goat anti-rabbit secondary antibody conjugated with HRP (Cell Signaling Technology, Inc., Denvers, MA). Signals were developed using the SuperSignal West Femto Substrate (Thermo Scientific) and detected by the FluoroChem digital imaging system (Cell Biosciences, Inc., Santa Clara, CA). The membrane was stripped, reblocked, and reprobed with mouse anti-β-actin antibody (Millipore) and goat anti-mouse secondary antibody conjugated with HRP (Santa Cruz Biotechnology, Inc.). Signals were developed using the same method as described previously.