Anterior segments of live animals were examined by high-power and fluorescent microscopy and by direct gonioscopy. Briefly, animals were anesthetized with intraperitoneal ketamine (#00409205310, 65 mg/kg; Hospira, Inc., Lake Forest, IL) and xylazine (#4821, 5 mg/kg; Lloyd, Inc., Shenandoah, IA); viscoelastic was applied to the eye before the animal was placed on a slide facing the objective of the inverted microscope. Intraocular pressure was measured in restrained animals with a rebound tonometer (iCare, Espoo, Finland) at the same time of day, taken in triplicate, and reported as the mean. As an indirect test for endothelial function and IOP confounder, pachymetric CCT (DGH Technology, Exton, PA) was measured after topical anesthesia (0.5% proparacaine; Alcon), obtained in triplicate, and reported as the mean.