Ciliary epithelium sections were incubated overnight at room temperature with different primary antibodies: rabbit anti-RhoA, anti-RhoB, or anti-Rac1 (1:200, 1:100, and 1:20, respectively; Santa Cruz, Dallas, TX, USA), sheep anti-Chx10 (1:100; Chemicon, Temecula, CA, USA), rabbit anti-Ki67 (1:100; Vector, Burlingame, CA, USA), rabbit anti-Pax6 (1:100; Babco/Covance, Princeton, NJ, USA), rat anti–proinflammatory markers CCL22 and CXCL11 (1:200; R&D Systems, Minneapolis, MN, USA), and rat antimicroglia marker Ox42 (1:300; Abcam, Cambridge, MA, USA), diluted in PB 0.1 M containing 3% normal donkey or goat serum, and 0.3% Triton X-100. After several washes in PB, sections were incubated for 2 hours with antisera against rabbit, sheep, or rat IgG (1:50), tagged to fluorescein isothiocyanate or rhodamine isothiocyanate (Jackson Laboratories, West Grove, PA, USA). Slides were then coverslipped with VectaShield (Vector Laboratories, Burlingame, CA, USA), analyzed with a Nikon PCM2000 (New York, NY, USA) or Zeiss LSM780 (Jena, Germany) confocal microscope. Figures were mounted with Adobe Photoshop (San Jose, CA, USA). Manipulation of the images was restricted to threshold and brightness adjustments to the whole image. Controls for the experiments consisted of the omission of primary antibodies; no staining was observed in these cases.