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Sonali Pal-Ghosh, Ahdeah Pajoohesh-Ganji, A. Sue Menko, Hye-young Oh, Gauri Tadvalkar, Daniel R. Saban, Mary Ann Stepp; Cytokine Deposition Alters Leukocyte Morphology and Initial Recruitment of Monocytes and γδT Cells After Corneal Injury. Invest. Ophthalmol. Vis. Sci. 2014;55(4):2757-2765. doi: 10.1167/iovs.13-13557.
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An in vivo mouse model reproducibly induces recurrent epithelial erosions in wild-type mice spontaneously 2 weeks after a single 1.5-mm corneal debridement wound made with a dulled blade. When 1.5-mm wounds are made by a rotating burr so that the corneal epithelial basement membrane is removed, corneas heal without developing erosions. Here, we characterize differences in cytokine deposition and changes in leukocytes between 0 and 6 hours after dulled-blade and rotating-burr wounding.
BALB/c mice were used to study 1.5-mm corneal wounds made using a dulled blade or a rotating burr. Mice were studied immediately after wounding (0 hour) and at 6 hours in vivo and in vitro in organ culture. Corneas, corneal extracts, and collagenase digests from naïve and wounded mice were used for three-dimensional (3D) confocal imaging, cytokine arrays, and flow cytometry.
Confocal imaging showed CD45, a protein derived from leukocytes, accumulates at the wound edge by 3 and 6 hours after wounding in vivo but not in vitro with more CD45 accumulating after dulled-blade compared with rotating-burr wounds. Morphologic changes occurred in CD45+ leukocytes and higher levels for several cytokines were detected in the stromal wound bed within minutes following dulled-blade wounds. Flow cytometry showed significantly more monocytes (CD45+/CD11b+/Ly6C+) and γδT cells (CD45+/GL3+) recruited into the corneas of mice with dulled-blade wounds by 6 hours.
Differences in cytokine-driven leukocyte responses are seen after dulled-blade debridement compared with rotating-burr injury.
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