November 1989
Volume 30, Issue 11
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Articles  |   November 1989
Local hypothermia protects the retina from ischemia. A quantitative study in the rat.
Author Affiliations
  • N Faberowski
    Duke University Medical Center, Department of Ophthalmology, Durham, North Carolina.
  • E Stefansson
    Duke University Medical Center, Department of Ophthalmology, Durham, North Carolina.
  • R C Davidson
    Duke University Medical Center, Department of Ophthalmology, Durham, North Carolina.
Investigative Ophthalmology & Visual Science November 1989, Vol.30, 2309-2313. doi:
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      N Faberowski, E Stefansson, R C Davidson; Local hypothermia protects the retina from ischemia. A quantitative study in the rat.. Invest. Ophthalmol. Vis. Sci. 1989;30(11):2309-2313.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

We developed a quantitative histologic method for assessing injury in the rat retina due to transient ischemia. We used this technique to test the effectiveness of local hypothermia and allopurinol, an inhibitor of oxygen-free radical formation, in reducing ischemia/reperfusion injury in the rat retina. Retinal ischemia and reperfusion was produced by transient ligation of the optic nerve. Histologic evaluation by a masked observer was based on the average count of nonpyknotic nuclei in the inner nuclear layer of the retina from eight high power fields (X100) in one 5 microns thick sagital section at or near the optic nerve. A sharp increase in tissue damage occurs between 90 and 120 min of ischemia. Ischemia for periods of 60 and 90 min produced mild damage while periods of 120 and 240 min produced severe damage. Hypothermia protected the retina significantly from 120 min of ischemic injury (P less than 0.001 student t-test, compared to 120 min control), while allopurinol had no protective effect.

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