February 1990
Volume 31, Issue 2
Free
Articles  |   February 1990
Glucose uptake, hexose monophosphate shunt activity, and oxygen consumption in cultured human retinal pigment epithelial cells.
Author Affiliations
  • M V Miceli
    Department of Ophthalmology, Louisiana State University Eye Center, New Orleans 70112.
  • D A Newsome
    Department of Ophthalmology, Louisiana State University Eye Center, New Orleans 70112.
  • G W Schriver
    Department of Ophthalmology, Louisiana State University Eye Center, New Orleans 70112.
Investigative Ophthalmology & Visual Science February 1990, Vol.31, 277-283. doi:
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      M V Miceli, D A Newsome, G W Schriver; Glucose uptake, hexose monophosphate shunt activity, and oxygen consumption in cultured human retinal pigment epithelial cells.. Invest. Ophthalmol. Vis. Sci. 1990;31(2):277-283.

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Abstract

Retinal pigment epithelium (RPE) was isolated from human donors with no known eye disease and normal-appearing fundi and was grown in culture. Population doubling times were measured for fifth passage RPE cells; the mean was 2.25 +/- 0.75 days and showed a correlation with the age of the donor. Metabolic studies were performed by perfusing the cells with 1-[13C]-D-glucose and monitoring the perfusate with magnetic resonance spectroscopy. Glucose uptake was 10.5 +/- 3.3 nmoles/min/mg protein. Lactate production from glucose was 20.0 +/- 5.00 nmoles/min/mg protein. The percentage of glucose through the hexose monophosphate shunt was 21 +/- 2%. Oxygen (O) consumption was measured at 20.1 +/- 4.1 nmoles O/min/mg endogenous and 14.5 +/- 4.8 nmoles O/min/mg with 10 mM glucose. These cells exhibit high rates of lactate production concomitantly with high oxygen consumptions and high flux through the hexose monophosphate shunt.

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