April 1990
Volume 31, Issue 4
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Articles  |   April 1990
Fibroblast behavior at aqueous interfaces with perfluorocarbon, silicone, and fluorosilicone liquids.
Author Affiliations
  • J R Sparrow
    Department of Ophthalmology, New York Hospital, Cornell University Medical Center, NY 10021.
  • R Ortiz
    Department of Ophthalmology, New York Hospital, Cornell University Medical Center, NY 10021.
  • P R MacLeish
    Department of Ophthalmology, New York Hospital, Cornell University Medical Center, NY 10021.
  • S Chang
    Department of Ophthalmology, New York Hospital, Cornell University Medical Center, NY 10021.
Investigative Ophthalmology & Visual Science April 1990, Vol.31, 638-646. doi:
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      J R Sparrow, R Ortiz, P R MacLeish, S Chang; Fibroblast behavior at aqueous interfaces with perfluorocarbon, silicone, and fluorosilicone liquids.. Invest. Ophthalmol. Vis. Sci. 1990;31(4):638-646.

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Abstract

Perfluorocarbon, silicone, and fluorosilicone liquids with potential for use as vitreous substitutes in the management of complex retinal detachment were evaluated for surface reactivity by assessing the behavior of anchorage-dependent fibroblasts plated at the phase boundary between these compounds and culture medium. Low-viscosity perfluorcarbons were alumina-treated to remove polar impurities. On perfluorodecalin, perfluorodimethylcyclohexane, perfluorotrimethylcyclohexane, perfluoroethylcyclohexane, perfluorooctane, perfluoroperhydrophenanthrene, perfluoromethyladamantane, perfluorodimethyladamantane, the highly viscous perfluoropolyether liquids Krytox TLF7067 and 6354, and dimethylsiloxane liquids of a variety of viscosities, most cells did not attach; the few that did attach exhibited minimal spreading behavior and did not achieve the flattened spindle-shape morphology which is a prerequisite to normal proliferative activity. Conversely, on perfluoromethyldecaline, perfluorofluorene, perfluorotributylamine, the perfluoropolyether K-6 hexamer, trifluoropropylmethylsiloxane (fluorosilicone), and diphenyldimethylsiloxane, some cells became fusiform-shaped and exhibited proliferation, the extent of which varied with the compound. The association of alumina treatment of perfluorocarbon liquids with a reduction in cell growth was indicative of a relationship between the presence of residual hydrogen-containing impurities and the capacity for cellular attachment and growth. This correlation was demonstrated also in experiments in which cell attachment and growth was facilitated by the addition of hydrogen-rich monohydroperfluorooctane to alumina-treated perfluorooctane. In conclusion, evidence for the presence of surface active impurities in liquid vitreous substitute materials can be obtained by observing the behavior of attachment-dependent cells plated at the boundary between these compounds and culture medium.

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