April 1990
Volume 31, Issue 4
Free
Articles  |   April 1990
Regional distribution of lysosomal enzymes in the canine retinal pigment epithelium.
Author Affiliations
  • L Cabral
    Department of Clinical Ophthalmology, Institute of Ophthalmology, London, United Kingdom.
  • W Unger
    Department of Clinical Ophthalmology, Institute of Ophthalmology, London, United Kingdom.
  • M Boulton
    Department of Clinical Ophthalmology, Institute of Ophthalmology, London, United Kingdom.
  • R Lightfoot
    Department of Clinical Ophthalmology, Institute of Ophthalmology, London, United Kingdom.
  • N McKechnie
    Department of Clinical Ophthalmology, Institute of Ophthalmology, London, United Kingdom.
  • I Grierson
    Department of Clinical Ophthalmology, Institute of Ophthalmology, London, United Kingdom.
  • J Marshall
    Department of Clinical Ophthalmology, Institute of Ophthalmology, London, United Kingdom.
Investigative Ophthalmology & Visual Science April 1990, Vol.31, 670-676. doi:
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      L Cabral, W Unger, M Boulton, R Lightfoot, N McKechnie, I Grierson, J Marshall; Regional distribution of lysosomal enzymes in the canine retinal pigment epithelium.. Invest. Ophthalmol. Vis. Sci. 1990;31(4):670-676.

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Abstract

We have investigated the activities of four lysosomal enzymes in RPE cells isolated from three regions of the canine fundus: the tapetal area, the central pigmented area and the peripheral area. The results obtained with freshly isolated cells showed that the activities of acid phosphatase, B-glucuronidase and N-acetyl-B-glucosaminidase were significantly higher in RPE cells derived from the peripheral region when compared to those from the two central regions. In contrast, the activity of cathepsin D was significantly higher in the tapetal region than in the periphery. The regional distribution of both acid phosphatase and B-glucuronidase observed in fresh RPE cells was progressively lost when these cells were grown in culture. Estimations of photoreceptor density per RPE cell from each of the regions indicated that the number of photoreceptors per RPE cell did not vary significantly with retinal location and suggested that variations in enzyme content were not related to differences in photoreceptor cell distribution.

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