September 1990
Volume 31, Issue 9
Free
Articles  |   September 1990
Effects of silicone oil and hyaluronic acid on cultured human retinal pigment epithelium.
Author Affiliations
  • T C Verstraeten
    Department of Ophthalmology, University of Pittsburgh School of Medicine, Pennsylvania.
  • D K Wilcox
    Department of Ophthalmology, University of Pittsburgh School of Medicine, Pennsylvania.
  • T R Friberg
    Department of Ophthalmology, University of Pittsburgh School of Medicine, Pennsylvania.
  • C Reel
    Department of Ophthalmology, University of Pittsburgh School of Medicine, Pennsylvania.
Investigative Ophthalmology & Visual Science September 1990, Vol.31, 1761-1766. doi:
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      T C Verstraeten, D K Wilcox, T R Friberg, C Reel; Effects of silicone oil and hyaluronic acid on cultured human retinal pigment epithelium.. Invest. Ophthalmol. Vis. Sci. 1990;31(9):1761-1766.

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Abstract

An experimental model was developed to evaluate the effects of several vitreous substitutes on human retinal pigment epithelial (RPE) cells. Cultured RPE cells were grown into confluent monolayers on semipermeable membranes and nourished from the basal side only, while the apical surface was directly and continuously exposed to preparations of hyaluronic acid and silicone oil for up to 4 weeks. In the silicone oil-treated group, multilayer sheets formed within 2 weeks of exposure and incorporation of 3H-thymidine was twice that of controls. Exposure of RPE cells to silicone oil and certain preparations of hyaluronic acid resulted in a three- to fourfold decrease in cellular polarity, as measured by assaying the cell-surface enzyme, gamma-glutamyl transpeptidase. These results suggest that a model using cultured RPE-cell monolayers is useful in identifying and understanding alterations in ocular tissues triggered by exposure to vitreous replacement materials.

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