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Corine Nicolas–Léveque, Ibtissem Ghedira, Jean Pierre Faure, Massoud Mirshahi; β-Arrestin–Related Proteins in Ocular Tissues. Invest. Ophthalmol. Vis. Sci. 1999;40(8):1812-1818.
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purpose. Proteins of the arrestin family contribute to the regulation of
G-protein–mediated transduction. In this study, the presence ofβ
-arrestins in ocular tissues was investigated.
methods. Mouse monoclonal and rabbit polyclonal antibodies were raised against
sequence present in β-arrestins 1 and 2 but absent from visual
arrestin. These antibodies were used for the immunohistologic detection
of β-arrestins in parafin sections of rodent eyes fixed in Bouin’s
solution. Reverse transcription–polymerase chain reaction (RT-PCR)
analysis of RNA from bovine retina, retinal pigmented epithelial (RPE)
cells, lens epithelial cells, and human corneal fibroblasts was
performed using β-1 arrestin primers.
results. In the eye, β-arrestin staining predominated in RPE, inner
segments of photoreceptors, synaptic spherules of rods, inner plexiform
layer and ganglion cell fibers, epithelial cells from ciliary body, and
vessels. RT- PCR amplified a 480 bp product, corresponding to the
predicted length. The sequence of PCR products from bovine retina and
RPE cells was identical with the bovine β-arrestin mRNA.
conclusions. β-arrestins were detected in several ocular tissues. In photoreceptor
cells, their specific localization in the synaptic terminals and
plexiform layer suggests a role of β-arrestin in synaptic
transmission. In other ocular tissues, the presence of β-arrestin may
be related either to adrenergic signal transduction or to signal
transduction mediated by other G-protein–coupled
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