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Catherine Creuzot–Garcher, Vincent Guerzider, Mahfoud Assem, Alain M. Bron, Philippe Delannoy, Jacques Bara; Alteration of Sialyl Lewis Epitope Expression in Pterygium. Invest. Ophthalmol. Vis. Sci. 1999;40(8):1631-1636.
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purpose. Mucin-related antigens are abundantly expressed by the cells of the
normal human conjunctiva. The pattern of these antigens in
pterygium, and especially the role of Galβ1-3GlcNAcα
2,3-sialyltransferase (ST3Gal III), sialyltransferase necessary to
build the sialyl-Lea (Lewisa) antigen, were
methods. Immunoperoxidase staining was performed on 28 pterygia using different
monoclonal antibodies: anti-M1 (against the peptidic core of gastric
mucins encoded by MUC 5AC gene),
anti-Lea (7LE), anti–sialyl Lea (NS
19-9), and anti-Leb (2-25LE). A serologic Lewis
determination was done in 18 patients. ST3Gal III sialyltransferase
expression was also studied in 10 healthy conjunctiva and 10 pterygia
by reverse transcriptase–polymerase chain reaction (RT–PCR).
Glyceraldehyde-3-phosphate-dehydrogenase was used as an endogenous
results. First, Lea, sialyl Lea, and Leb immunoreactivities either decreased or were no longer detectable in
pterygium goblet cells as opposed to normal conjunctiva. Second, unlike
in pterygium, the Lewis immunoreactivity, which is mainly located in
the surface epithelial cells in the normal conjunctiva, was
occasionally restricted to the epithelial cells of the deep layers.
However, M1 mucins did show an identical pattern expression in a normal
conjunctiva and pterygium. ST3Gal III expression was significantly
lower in pterygium (0.20 ± 0.02 AU [arbitrary units]) than in
normal conjunctiva (0.95 ± 0.12 AU).
conclusions. ST3Gal III gene is less expressed in pterygium than in normal
conjunctiva. This observation could explain the decrease of sialyl
Lea expression observed in pterygium by immunohistology.
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