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Jun Shimazaki, Minako Kaido, Naoshi Shinozaki, Shigeto Shimmura, Batmunkh Munkhbat, Masao Hagihara, Kimiyoshi Tsuji, Kazuo Tsubota; Evidence of Long-term Survival of Donor-Derived Cells after Limbal Allograft Transplantation. Invest. Ophthalmol. Vis. Sci. 1999;40(8):1664-1668.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. Severe destruction of the corneal limbus causes conjunctival invasion
and subsequent visual loss. Limbal allograft transplantation (LAT) was
recently proposed for the treatment of these disorders. However,
whether the method functions as a stem cell transplantation of the
corneal epithelium remains unclear. This study provided evidence that
donor-derived corneal epithelial cells survive long after LAT.
methods. Epithelial cells on the paracentral cornea in patients who have
undergone LAT were subjected to fluorescence in situ hybridization
(FISH) and polymerase chain reaction restriction fragment length
polymorphism (RFLP) analysis. X and Y chromosomes were detected using
sex chromosome–specific probes in the FISH analysis, and HLA-DPB1
antigens were examined in the RFLP analysis. Eyes receiving
conventional penetrating keratoplasty (PKP) served as controls.
results. Donor-derived epithelial cells were detected in three of five eyes
(60.0%) in the FISH analysis and in seven of nine eyes (77.8%) in the
RFLP analysis. Among these eyes, one and three eyes in the FISH and
RFLP analysis, respectively, had both donor- and recipient-derived
cells. In control PKP eyes, none of the eyes in the FISH analysis and
one of eight eyes (12.5%) in the RFLP analysis had donor-derived
conclusions. These results suggest that donor-derived cells survive much longer
after LAT than those after PKP, and that LAT may function as stem cell
transplantation of the corneal epithelium.
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