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Ming Lu, Victor L. Perez, Naili Ma, Kazuaki Miyamoto, Hai–Bing Peng, James K. Liao, Anthony P. Adamis; VEGF Increases Retinal Vascular ICAM-1 Expression In Vivo. Invest. Ophthalmol. Vis. Sci. 1999;40(8):1808-1812.
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purpose. Intraocular injections of vascular endothelial growth factor (VEGF), a
peptide implicated in the pathogenesis of diabetic retinopathy, can
induce retinal ischemia. Diabetic retinal ischemia may be caused, in
part, by the adhesion of leukocytes to the retinal vasculature. In this
study, the ability of VEGF to increase the expression of intercellular
adhesion molecule-1 (ICAM-1) and other adhesion molecules in capillary
endothelium and the retinal vasculature was examined.
methods. The expression of ICAM-1, vascular cell adhesion molecule-1 (VCAM-1),
E-selectin, and P-selectin on human brain capillary endothelial cell
monolayers exposed to VEGF was quantitated by immunoassay. The effect
of VEGF on retinal vascular ICAM-1 expression was determined in ICAM-1
immunofluorescence studies of retinal flat-mounts and in RNase
results. VEGF increased capillary endothelial cell ICAM-1 levels in a dose- and
time-dependent manner (6–24 hours, plateau after 6 hours;
EC50, 25 ng/ml). VEGF failed to alter E-selectin,
P-selectin, or VCAM-1 levels under the conditions tested. Intravitreal
injections of pathophysiologically relevant concentrations of VEGF
increased ICAM-1 protein and mRNA levels in the retinal vasculature.
conclusions. VEGF increases retinal vascular ICAM-1 expression. VEGF-induced
increases in ICAM-1 may promote retinal leukostasis in diabetic eyes.
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