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Hans-Peter Hammes, Hans Hoerauf, Alex Alt, Erwin Schleicher, Jes Thorn Clausen, Reinhard G. Bretzel, Horst Laqua; Nε(Carboxymethyl)Lysin and the AGE Receptor RAGE Colocalize in Age-Related Macular Degeneration. Invest. Ophthalmol. Vis. Sci. 1999;40(8):1855-1859.
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purpose. To investigate whether glycoxidation products and the receptor for
advanced glycation end products (RAGE) are present and colocalize in
subfoveal membranes of patients with age-related macular degeneration
methods. Surgically removed subfoveal fibrovascular membranes from 12 patients,
11 related to ARMD and 1 to an idiopathic membrane, were analyzed for
the presence of the glycoxidation product
Nε-(carboxymethyl)lysin (CML), one of the receptors for
advanced glycation end products, RAGE, and the activation of NFkB,
results. CML-like immunoreactivity was found in all ARMD specimens examined
adjacent or colocalized with RAGE, but not in the idiopathic membrane.
RAGE immunoreactive material was found in CD68-positive cells and in
the fibrous matrix. CD68-positive cells and surrounding areas stained
for p50, the activated form of NFkB.
conclusions. These results indicate that glycoxidation products are present in
subretinal membranes of patients with ARMD. The concomitant expression
of RAGE in these membranes and the finding of activated NFkB is
suggestive of an implication of glycoxidation product formation in the
pathogenesis of the disease.
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