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Yoritsugu Hada, Yuko Yamada, Kazuyuki Imamura, Nobuko Mataga, Yasuyoshi Watanabe, Misao Yamamoto; Effects of Monocular Enucleation on Parvalbumin in Rat Visual System during Postnatal Development. Invest. Ophthalmol. Vis. Sci. 1999;40(11):2535-2545.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. To re-evaluate the hypothesis that the expression of the
calcium-binding protein parvalbumin (PV) in a subpopulation ofγ
-aminobutyric acid (GABA)ergic neurons is an appropriate molecular
marker for the effect on ocular dominance plasticity of monocular
deprivation during the postnatal sensitive period.
methods. Long–Evans rats underwent monocular enucleation immediately before eye
opening (postnatal day [P] 14). Immunohistochemical analysis using
anti-PV antibody was performed on the superior colliculus (SC) and
lateral geniculate nucleus (LGN) at P45. In the visual cortex (VC)
developmental changes in immunoreactivity were also examined at the
ages of P17, P20, P27, and P45. Northern blot analysis for PV mRNA was
also performed at P45. Changes in PV expression in the visual system of
these rats were evaluated by use of a computer-based quantitative
results. PV-immunoreactive neurons were present in the SC and VC, whereas only a
few were found in the LGN. The monocular enucleation at the onset of
the sensitive period markedly reduced PV immunoreactivity in the
neuropil of the SC, contralateral to the enucleated eye when examined
one month later. No consistent and significant change in PV
immunoreactivity was found in either the LGN or the VC. The number of
PV-immunoreactive neurons in the VC rapidly decreased to the adult
level during the middle of the sensitive period. The expression of PV
mRNA in these central visual structures was not affected by early
conclusions. Expression of PV is developmentally regulated, and marked changes in
its protein expression in the SC can be induced by monocular
enucleation. Contrary to the original hypothesis, monocular enucleation
did not consistently affect the expression of PV in the rat VC. The
expression of PV is probably regulated by multiple factors, not merely
by binocular competition.
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