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Cheol Hwa Song, Jae Sin Choi, Dae Kyong Kim, Jae Chan Kim; Enhanced Secretory Group II PLA2 Activity in the Tears of Chronic Blepharitis Patients. Invest. Ophthalmol. Vis. Sci. 1999;40(11):2744-2748.
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purpose. Phospholipase A2 (PLA2) hydrolyzes
phospholipids, one of the important constituents of human meibomian
gland secretions. This study was performed to investigate
PLA2 type and activity in the tears of chronic blepharitis
patients compared to those of normal persons.
methods. Tear samples of 36 patients and 10 normal persons were collected in
non-heparinized microcapillary tubes. PLA2 activity in the
tears was measured by Dole’s method, and the results of the
blepharitis patients were compared to those of the normal persons. The
characterization of PLA2 was performed by the head group
preference test and the dithiothreitol (DTT) sensitivity test. The
classification of PLA2 type was done using Western blot
analysis with anti-human secretory PLA2 antibody.
results. No statistically significant differences were found among the six
categories of chronic blepharitis. However, the mean PLA2 activity in the tears of the chronic blepharitis patients was about two
times higher than that of the normal controls with statistical
significance (P < 0.05). The PLA2 substrate
specificity test revealed group II PLA2 activity.
Furthermore, the group II PLA2 was identified as a 14 kDa
band in Western blot analysis using an antibody raised against human
secretory group II PLA2.
conclusions. Secretory group II PLA2 activity was significantly enhanced
in the tears of the chronic blepharitis patients compared with that of
the normal controls. It is suggested that this increased enzymatic
activity may decrease the tear film stability through increased
hydrolysis of phospholipids.
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