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Takeshi Sagara, Dan D. Gaton, James D. Lindsey, B’Ann True Gabelt, Paul L. Kaufman, Robert N. Weinreb; Reduction of Collagen Type I in the Ciliary Muscle of Inflamed Monkey Eyes. Invest. Ophthalmol. Vis. Sci. 1999;40(11):2568-2576.
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purpose. To investigate in monkey ciliary muscle the relationship between the
extent of anterior segment inflammation and alterations of collagen
type I as determined by quantitative imaging densitometry.
methods. Anterior segment inflammation was induced in one eye of five cynomolgus
monkey by cannulation of the anterior chamber, by anterior chamber
injection of bovine serum albumin, or by disruption of the iris and
anterior lens capsule with a needle. Increases in inflammatory cells
were scored in hematoxylin and eosin–stained sections. Parallel eye
sections were immunostained for collagen type I and developed using
diaminobenzidine. Optical density (OD) was measured along two line
segments overlying the immunostained ciliary muscle using
two-dimensional imaging densitometry. To assess antibody labeling of
ciliary muscle structures, additional sections were
double-immunostained using antibodies to collagen type I and calponin
and examined by confocal microscopy.
results. In each of the inflamed eyes, hematoxylin and eosin–stained sections
showed signs of chronic inflammation including lymphocytes and
macrophages dispersed among ciliary muscle fibers and in the iris.
Double label confocal microscopy showed collagen type I
immunoreactivity in the interstitial extracellular matrix between
bundles of ciliary smooth muscle fibers. Collagen type I OD scores in
each of the inflamed eyes were less by 16% to 55%, compared with the
contralateral control eyes. The mean of the OD scores for all inflamed
eyes was 39% ± 7% less than the mean of the control eye scores
(mean ± SEM, P < 0.001). Regression analysis
showed a close correlation between inflammatory cell scores in the
treated eyes and the reduction of OD scores (r =
0.94, P = 0.02).
conclusions. These results indicate that the density of collagen type I in
the extracellular matrix (ECM) of monkey ciliary muscle is reduced
during anterior segment inflammation and support the view that
reduction of ciliary muscle ECM may contribute to increased uveoscleral
outflow facility during anterior segment
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