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Shannon Saszik, Joseph Bilotta; Effects of Abnormal Light-rearing Conditions on Retinal Physiology in Larvae Zebrafish. Invest. Ophthalmol. Vis. Sci. 1999;40(12):3026-3031.
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purpose. Anatomic studies have found that zebrafish retinal neurons
develop in a sequential fashion. In addition, exposure to abnormal
light-rearing conditions produces deficits in visual behavior of larvae
zebrafish, even though there appears to be little effect of the
light-rearing conditions on the gross morphology of the retina. The
purpose of this study was to assess the effects of abnormal
light-rearing conditions on larvae zebrafish retinal physiology.
methods. Larvae zebrafish (Danio rerio) were exposed to constant
light (LL), constant dark (DD), or normal cyclic light (LD) from
fertilization to 6 days postfertilization (dpf). After 6 days, the
animals were placed into normal cyclic light and tested at 6 to 8, 13
to 15, and 21 to 24 dpf. Electroretinogram (ERG) responses to visual
stimuli, consisting of various wavelengths and irradiances, were
recorded. Comparisons were made across the three age groups and the
three light-rearing conditions.
results. Deficits from the light-rearing conditions were seen immediately after
exposure (6–8 dpf). The LL-condition subjects showed the greatest
deficit in the UV and short-wavelength areas and the DD-condition
subjects showed a slight deficit across the entire spectrum. At 13 to
15 dpf, the LL and DD groups showed an increase in sensitivity and by
21 to 24 dpf, the groups no longer differed from controls.
conclusions. Abnormal lighting environments can adversely influence the
physiological development of the larvae zebrafish retina. The pattern
of damage that was seen in zebrafish is similar to that found in other
vertebrates, including higher vertebrates. However, unlike higher
vertebrates, the zebrafish appears to be capable of regeneration. This
suggests that the zebrafish would be a viable model for light
environment effects and neural regeneration.
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