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David E. Rudnick, Jeremy S. Noonan, Dayle H. Geroski, Mark R. Prausnitz, Henry F. Edelhauser; The Effect of Intraocular Pressure on Human and Rabbit Scleral Permeability. Invest. Ophthalmol. Vis. Sci. 1999;40(12):3054-3058.
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purpose. The purpose of this study was to evaluate the effects of intraocular
pressure on the permeability of human and rabbit sclera to water,
dexamethasone, and carboxyfluorescein.
methods. Scleral sections excised from moist-chamber–stored human globes or
eyes obtained from euthanatized New Zealand White rabbits were mounted
in a perfusion chamber that can create a transscleral pressure that
simulates an intraocular pressure. A small depot of drug (100 μl) was
added to the episcleral surface while perfusing an irrigating solution
slowly across the choroidal side. The perfusate was collected and
scleral permeability calculated. Experiments were performed at 0, 15,
30, and 60 mm Hg for each compound in human and rabbit tissue.
results. Analysis of variance showed a significant effect of intraocular
pressure on both human and rabbit scleral permeability. Human scleral
permeability was decreased by as much as a factor of two for water
(P = 0.0004), dexamethasone (P <
0.0001), and carboxyfluorescein (P = 0.0064) at
elevated intraocular pressures. Rabbit scleral permeability was
similarly affected by elevated intraocular pressure for water
(P = 0.0039), dexamethasone (P = 0.0001), and carboxyfluorescein (P = 0.0016).
conclusions. This study shows that simulated intraocular pressure ranging from 15 to
60 mm Hg can decrease scleral permeability to small molecules by one
half when compared with the sclera with no pressure
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