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Steven Nusinowitz, William H. Ridder, John R. Heckenlively; Rod Multifocal Electroretinograms in Mice. Invest. Ophthalmol. Vis. Sci. 1999;40(12):2848-2858.
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purpose. To test the feasibility of recording rod multifocal electroretinograms
(ERGs) from the mouse eye.
methods. Multifocal ERGs were recorded from normal mice (C57BL/6J) using an
array of equal-sized hexagons. Local stimuli were blue (W47A), and the
number of blank frames between successive flashes at the same location
was fixed at 14 (minimum 200 msec between flashes). Flash and surround
intensity, and the number of hexagons, were varied to optimize the
stimulus conditions for the mouse, and alterations in adaptation level
were used to assess cone intrusion. Local response isolation was
evaluated by comparing multifocal responses to full-field ERGs and by
mapping local defects in laser-treated mice.
results. Rod multifocal ERGs, although small, were clearly recordable and well
formed under many conditions. Decreasing flash intensity or the size of
stimulus elements, and/or increasing the surround intensity or
adaptation level, decreased local response amplitudes. At the dimmest
flash intensity (−0.70 log scotopic trolands [scot td]/s) and the
smallest stimulus element (2.9° × 3.5°), local responses were
nondetectable. Comparisons with full-field ERGs supported the
hypothesis that the local responses were not contaminated by
contributions from dark-adapted retinal areas surrounding the
multifocal display. With sufficiently bright (0.30 log scot td-s) and
relatively large (5.6° × 6.9°) stimulus elements, multifocal
responses clearly revealed local retinal defects created with laser
conclusions. Rod multifocal ERGs can be recorded from the mouse eye to provide
topographical maps of retinal function that have sufficient spatial
resolution to be of practical use. The technique will be useful in
characterizing the natural history of regional loss in mouse models of
human retinal disease and in evaluating some forms of interventional
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