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Stephen E. Jones, Catherine Jomary, John Grist, Jayneeta Makwana, Michael J. Neal; Retinal Expression of γ-Crystallins in the Mouse. Invest. Ophthalmol. Vis. Sci. 1999;40(12):3017-3020.
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purpose. High levels of expression of a form of γ-crystallin mRNA in
mouse retina have been identified. Because the six murineγ
-crystallins have generally been regarded as specific to the lens,
the expression of these crystallins at the mRNA and protein levels in
the retina were evaluated in more detail.
methods. Expression of γE/F-crystallin mRNA was examined by northern blot
analysis and reverse transcription–polymerase chain reaction (RT–PCR)
analysis applied to murine retinal and lens total RNAs. Forγ
A-D-crystallin mRNAs, a multiplex RT–PCR was used on total cDNAs.
The detection of total γ-crystallin protein in the retina was
performed using an antibody to bovine lens γ-crystallins, applied to
protein extracts in immunoblot analysis and to cryostat sections of
ocular tissues in immunofluorescence studies.
results. By RT–PCR, we confirmed expression of both γE- and γF-crystallin
as well as all four (γA–γD) remaining crystallins at the mRNA
level in the mouse retina. γ-Crystallin proteins were also detectable
in murine retina by immunoblot analysis, although at a lower level than
in the lens. By immunocytochemistry, γ-crystallins were localized
particularly to the inner retina, outer plexiform layer, and the
photoreceptors during postnatal development.
conclusions. Our findings of γ-crystallin mRNA and protein expression in the
retina indicate that none of the major crystallin classes is uniquely
expressed in the lens. The expression of γ-crystallins in the
developing murine retina suggests a role analogous to the anti-stress
properties established for the small heat-shock proteinα
B-crystallin, perhaps in response to varying exposure to
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