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Maria Antonietta Blasi, Vittoria Maresca, Mariolina Roccella, Francesca Roccella, Tiziana Sansolini, Paola Grammatico, Emilio Balestrazzi, Mauro Picardo; Antioxidant Pattern in Uveal Melanocytes and Melanoma Cell Cultures. Invest. Ophthalmol. Vis. Sci. 1999;40(12):3012-3016.
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purpose. To investigate the antioxidant status of cultured uveal melanocytes from patients with
uveal melanoma and uveal melanoma cells to characterize some of the
biochemical properties of these cells in respect to the normal
methods. The fatty acid pattern of membrane phospholipids, intracellular vitamin
E level, and superoxide dismutase (SOD) and catalase activities were
studied in uveal melanocytes (n = 10) and uveal
melanoma cell (n = 10) cultures, by gas
chromatography mass spectrometry or by spectrophotometer.
results. Among the uveal melanocyte cultures, two groups were
differentiated, according to catalase activity: group A with catalase
values comparable to those of cutaneous ones and higher SOD activity
and group B with catalase values 2 SD lower (P <
0.001) and lower SOD activity. Vitamin E concentration was not
significantly different between melanoma cells and melanocytes, whereas
a significantly higher percentage of polyunsaturated fatty acids was
found in melanoma cells and the B group of melanocytes
(P = 0.022). In uveal melanoma cells SOD
activity was significantly lower than that detected in uveal
melanocytes (P < 0.005).
conclusions. These results show a different pattern of antioxidants in uveal
melanocytes with respect to cutaneous ones, possibly related to the
anatomic distribution. However, as in cutaneous melanocytes, two
subgroups were identified on the basis of the antioxidant pattern that
could be the expression of a constitutional increased susceptibility to
oxidative stress in some subjects. Moreover, an imbalance of the
antioxidants was observed in melanoma cells, possibly related to the
disease status and progression.
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