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Akitaka Tsujikawa, Junichi Kiryu, Atsushi Nonaka, Kenji Yamashiro, Hirokazu Nishiwaki, Shinichiro J. Tojo, Yuichiro Ogura, Yoshihito Honda; In Vivo Evaluation of Platelet–Endothelial Interactions in Retinal Microcirculation of Rats. Invest. Ophthalmol. Vis. Sci. 1999;40(12):2918-2924.
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purpose. This study was designed to develop a new method to evaluate the
dynamics of platelets in the retinal microcirculation in vivo and to
investigate quantitatively the platelet–endothelial interactions in
rat retina with the use of this system.
methods. Isolated platelet samples were labeled with carboxyfluorescein
diacetate succinimidyl ester. After intravenous administration,
platelet behavior in the retinal microcirculation was evaluated with a
scanning laser ophthalmoscope. The images were recorded on S-VHS
videotape and analyzed with a computer-assisted image analysis system.
The platelet–endothelial interactions in the retinal microcirculation
were also investigated with the use of lipopolysaccharide-stimulated
endothelium or platelets activated with thrombin.
results. Fluorescent platelets were recognized as distinct dots in the retinal
microcirculation and could be traced frame by frame. The velocity of
platelets in the retinal arteries, capillaries, and veins was 26.1 ± 6.4, 1.6 ± 0.4, and 19.9 ± 8.2 mm/sec, respectively. In
control rats, even the activated platelets showed minimal interaction
with retinal endothelial cells. In contrast, stimulated retinal
endothelium showed active platelet–endothelial interactions; many
platelets were observed rolling and adhering along the major retinal
veins. The interactions between platelets and stimulated endothelial
cells were substantially inhibited with the injection of P-selectin
conclusions. The present study demonstrated a new method to visualize platelet
behavior in the retinal microcirculation in vivo. This method will
allow quantitative evaluation of platelet dynamics and
platelet–endothelial interactions in retinal pathologic
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