November 1999
Volume 40, Issue 12
Letters to the Editor  |   November 1999
Normal Rat Retina Lacks Significant Numbers of ED2-Positive Macrophages
Author Affiliations
  • Peizeng Yang
    1Department of Ophthalmo-Immunology, The Netherlands Ophthalmic Research Institute, Amsterdam;
    2Zhongshan Ophthalmic Centre, Guangzhou, P.R. China;
  • Alex F. de Vos
    1Department of Ophthalmo-Immunology, The Netherlands Ophthalmic Research Institute, Amsterdam;
  • Aize Kijlstra
    1Department of Ophthalmo-Immunology, The Netherlands Ophthalmic Research Institute, Amsterdam;
    3Department of Ophthalmology, The University of Amsterdam, The Netherlands
Investigative Ophthalmology & Visual Science November 1999, Vol.40, 3067. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Peizeng Yang, Alex F. de Vos, Aize Kijlstra; Normal Rat Retina Lacks Significant Numbers of ED2-Positive Macrophages. Invest. Ophthalmol. Vis. Sci. 1999;40(12):3067.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements
We want to correct some statements that we published in an earlier volume of IOVS. 1 We described microglia and macrophages in the normal rat retina and the response of these cells during the endotoxin-induced uveitis model (EIU). Retinal wholemounts from normal rats and at various time points after endotoxin injection were stained with the monoclonal antibodies ED1 and ED2. We reported the staining pattern and densities (196–271 cells/mm2 ) of these two antibodies to be essentially similar and described a massive influx of macrophages 24 to 96 hours after endotoxin injection. We reported that these cells were both ED1+ and ED2+. Since then we have repeated the experiments and performed similar protocols in collaboration with Dr. Paul McMenamin, who has extensive experience with these monoclonal antibodies, during a sabbatical visit he paid to our Institute. We experienced some difficulties in the staining of ED2, which caused us to review the material originally published in IOVS. With the aid of Dr. McMenamin, we performed extensive tests with the monoclonal antibody batches used in the original experiments, compared them with new batches, and concluded that retinal microglia, i.e., the macrophages within the retinal parenchyma, are weakly ED1+ but ED2−. The original findings that these cells were ED2+ was in fact erroneous and appeared to be due to an error in labeling of antibodies at the time of aliquoting. It is important that this issue be clarified, because a number of studies of normal rat retinal tissue have failed to find significant numbers of ED2+ macrophages in the retina. 2 3 Furthermore, no influx of ED2+ macrophages was noted in EIU by Pouvreau et al. 2 We apologize for any inconvenience caused to our colleagues as a result of this error. 
Yang P, de Vos A, Kijlstra A. Macrophages in the retina of normal Lewis rats and their dynamics after injection of LPS. Invest Ophthalmol Vis Sci. 1996;37:77–85. [PubMed]
Pouvreau I, Zech JC, Thillaye-Goldenberg B, Naud MC, Van Rooijen N, de Kozak Y. Effect of macrophage depletion by liposomes containing dichloromethylene-diphosphonate on endotoxin-induced uveitis. J Neuroimmunol. 1998;86:171–181. [CrossRef] [PubMed]
Akaishi K, Ishiguro S, Durlu YK, Tamai M. Quantitative analysis of major histocompatibility complex class II-positive cells in posterior segment of Royal College of Surgeons rat eyes. Jpn J Ophthalmol. 1998;42:357–362. [CrossRef] [PubMed]

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.