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Hartmut Wenkel, David Kent, Paul Hiscott, Mark Batterbury, Carl Groenewald, Carl M. Sheridan, Lu–Gang Yu, Jeremy Milton; Modulation of Retinal Pigment Epithelial Cell Behavior by Agaricus Bisporus Lectin. Invest. Ophthalmol. Vis. Sci. 1999;40(12):3058-3062.
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purpose. To determine whether Agaricus bisporus lectin (ABL) binds
retinal pigment epithelial cells (RPEs), to conduct a preliminary
viability study of RPEs exposed to ABL, and to evaluate the effects of
ABL on RPE proliferation and RPE-mediated matrix contraction in vitro.
methods. Using cultured bovine RPEs, immunohistochemistry was used to study ABL
binding. Morphologic and trypan blue exclusion techniques were used for
toxicity studies. The effect of ABL on RPE proliferation was
investigated by [methyl-3H]–thymidine incorporation. The
effect of ABL on RPE-mediated matrix contraction was evaluated with
RPE-populated three-dimensional collagen matrices.
results. ABL bound to RPE cells. This binding was inhibited by asialomucin. No
change in RPE morphology or trypan blue exclusion compared with
controls was observed in RPEs incubated with 5 to 60 μg/ml ABL for 3
days. Twenty-four-hour incubations of RPEs with ABL significantly
inhibited RPE proliferation in a dose-dependent way, 40 μg/ml ABL
inhibited proliferation by 83% (SE 14, P < 0.05).
ABL showed a dose-dependent significant inhibition of RPE-mediated
collagen matrix contraction over 3 days, with 93% inhibition compared
with controls by 40 μg/ml lectin (P < 0.05). The
inhibitory effect of ABL on proliferation and gel contraction was
partly reversible after eliminating ABL from the culture medium.
conclusions. Bovine RPE cells bind ABL, and preliminary evaluations suggest that
levels of ABL that are nontoxic to the cells potently inhibit RPE
proliferation and RPE-mediated matrix contraction. ABL deserves further
investigation as a potential inhibitor of RPE proliferation and
cell-mediated matrix contraction in anomalous reparative processes such
as proliferative vitreoretinopathy and as a laboratory tool for RPE
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