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Indira Mamballikalathil, Charles Mann, Clyde Guidry; Tractional Force Generation by Porcine Müller Cells: Paracrine Stimulation by Retinal Pigment Epithelium. Invest. Ophthalmol. Vis. Sci. 2000;41(2):529-536.
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purpose. To examine the ability of retinal pigment epithelial (RPE) cells to
modulate Müller cell extracellular matrix contraction through
methods. Freshly isolated RPE cells were maintained in continuous culture until
the morphologic and immunocytochemical changes associated with
myofibroblastic dedifferentiation were complete. Secretory products
collected from these cells during extended incubations in serum-free
medium and at different stages of dedifferentiation were examined for
the ability to promote extracellular matrix contraction by Müller
cells. The contributions of specific growth factors to RPE-secreted
activity were examined with growth factor–neutralizing antibodies.
results. Secretory products from RPE cells throughout dedifferentiation
contained biologically active quantities of Müller cell
contraction promoters. Secretory activity increased during extended
incubation in serum-free medium and during myofibroblastic
dedifferentiation. Growth factor–specific neutralizing antibodies
enabled the determination that insulin-like growth factor– and
platelet-derived growth factor–related proteins were the secreted
species to which Müller cells responded. Finally, gene expression
of insulin-like growth factor 1 and platelet-derived growth factor A
chain by porcine RPE cells was confirmed using reverse
transcription-polymerase chain reaction.
conclusions. RPE cells are a viable source of biologically active quantities of two
growth factors that stimulate extracellular matrix contraction by
Müller cells. This secretory profile persists for extended
periods in an otherwise serum-free environment and is enhanced during
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