Purchase this article with an account.
Laurent Castelnovo, Christine Dosquet, Alain Gaudric, José Sahel, David Hicks; Human Platelet Suspension Stimulates Porcine Retinal Glial Proliferation and Migration In Vitro. Invest. Ophthalmol. Vis. Sci. 2000;41(2):601-609.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To characterize the cellular and molecular mechanisms underlying the
efficacy of autologous platelet suspension adjuvant therapy in the
treatment of macular hole.
methods. Platelet suspensions were prepared from whole blood samples obtained
from informed volunteers. For proliferation assays, platelet
suspensions or purified growth factors were added to semi-confluent
cultures of porcine retinal glial cells for 24 hours, followed by[ 3H]thymidine for 15 hours, after which time cells were
washed, solubilized, and counted for uptake of radioactive tracer. For
cell migration assays, confluent glial cultures were scrape wounded and
maintained in the presence or absence of platelet suspension or
identified platelet constituents. Cell migration into the denuded area
was scored as a function of time. In certain cases, specific
pharmacologic inhibitors of growth factor action were added at the same
time as platelet adjuvant or growth factors.
results. Platelet suspension adjuvant induced strong mitogenic and chemotactic
responses in cultured glia, in a dose-dependent manner. Maximal
incorporation of thymidine was two- to threefold that of control
levels, with an ED50 ∼5 × 106 platelets/ml, and migration was enhanced up to 80-fold after 48 hours.
Platelet suspension-induced proliferation was completely blocked by
addition of 25 μM genistein, a tyrosine kinase receptor inhibitor.
However, the same concentration only partially blocked the cell
migration response. Addition of any single growth factor or protein
identified from ELISA analysis, or a combination of all factors, did
not significantly stimulate proliferation or cell migration.
conclusions. Human platelet suspensions exert both proliferative and chemotactic
influences on retinal glial cells in vitro, suggesting that the same
responses may occur in platelet-induced macular hole repair in humans.
Growth factors or proteins that have been identified within the
suspensions do not mimic these responses in vitro, implying that
additional currently unidentified trophic activities are also
This PDF is available to Subscribers Only