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Valery I. Shestopalov, Steven Bassnett; Three-Dimensional Organization of Primary Lens Fiber Cells. Invest. Ophthalmol. Vis. Sci. 2000;41(3):859-863.
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purpose. To visualize the three-dimensional organization of primary lens fiber
methods. The gene for Green Fluorescent Protein (GFP) was introduced into the
lens vesicle using two different vector systems: a replication
deficient adenovirus or an expression plasmid. Injected embryos were
allowed to develop for several days and then were examined by confocal
results. Injection of either vector resulted in GFP expression in primary fiber
cells. GFP-expressing cells were heterogeneous in shape and length.
Some regions of the fibers were varicose, with diameters >10 μm;
regions between the varicosities were often extremely thin, with
diameters of <2 μm. No differences in the morphologies of
GFP-expressing cells were noted between adenovirus- and
plasmid-injected lenses, suggesting that the irregular, undulating,
appearance of the primary fibers was not the result of viral infection.
Three-dimensional reconstruction of primary fiber cells revealed that,
by E6, the posterior tips of the fibers had detached from the lens
capsule. The anterior fiber tips remained in contact with the overlying
epithelium for 1 to 2 additional days, demonstrating that the formation
of the anterior and posterior sutures was asynchronous.
conclusions. The three-dimensional cellular organization of GFP-expressing cells is
consistent with previous analyses of fiber cell morphology in the
embryonic nucleus of adult human and bovine lenses. The present data
confirm that the disorganized appearance of primary fiber cells
observed in adult lenses is largely a reflection of developmental
processes rather than a consequence of aging.
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