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Linda D. Hazlett, Sharon McClellan, Byungsuk Kwon, Ronald Barrett; Increased Severity of Pseudomonas aeruginosa Corneal Infection in Strains of Mice Designated as Th1 versus Th2 Responsive. Invest. Ophthalmol. Vis. Sci. 2000;41(3):805-810.
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purpose. Mice favoring Th1 (C57BL/6, C57BL/10, and B10.D2/nSn) versus Th2
(BALB/c, BALB/cBy, BALB.B, and BALB.K) response development were
evaluated for their response to infection with Pseudomonas
aeruginosa. This study addresses the question of whether Th1
versus Th2 response propensity affects the pathogenesis of bacterial
keratitis in mice.
methods. Ocular disease was determined by mean clinical score, slit lamp, plate
counts, and histopathology, and antigen-specific cellular responses
were assessed by immunostaining and measurement of delayed type
results. Strains of mice favoring Th1 (B6, BL10, and B10.D2) versus Th2 (BALB/c,
BALB/cBy, BALB.B, and BALB.K) responsiveness were infected with P. aeruginosa. Mice favoring Th1 response development
exhibited a similar course of disease and the infected eyes of all mice
perforated by 7 days postinfection (p.i.). Strains (BALB/c, BALB/cBy,
BALB.B, and BALB.K) favoring Th2 response development exhibited a
milder course of disease, and none of the infected corneas perforated
at 7 days p.i. In a Th1-responsive strain (B10.D2), positive
immunostaining for CD4+ and CD8+ T cells was
observed in the cornea by 3 days p.i. and by 5 days p.i., respectively,
some cells stained positively for IL2-R, indicating that the cells were
activated. In contrast, in a Th2 responder strain (BALB/c), there was
no detectable positive immunostaining in cornea for any of the T-cell
markers tested and DTH was significantly elevated in B10.D2 versus
conclusions. These studies are the first to provide evidence that in P.
aeruginosa ocular infection, mouse strains favoring development
of a Th1-type response are susceptible (cornea perforates), whereas
strains favoring Th2 response development are resistant (no corneal
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