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Richard S. Berk, Malkhan Katar, Zhong Dong, Duane E. Day; Plasminogen Activators and Inhibitors in the Corneas of Mice Infected with Pseudomonas aeruginosa. Invest. Ophthalmol. Vis. Sci. 2001;42(7):1561-1567. doi: https://doi.org/.
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purpose. To characterize the presence of plasminogen activators and their
inhibitors in the corneas during the inflammatory response in
naïve and immunized mice intracorneally infected with Pseudomonas aeruginosa.
methods. RT-PCR was used to detect gene expression for plasminogen activators
and their inhibitors in naïve and immunized mice. Immunoblot
analysis, zymography, and ELISA were used to demonstrate the syntheses
of these proteins.
results. Naïve mice intracorneally infected with P.
aeruginosa showed a temporally enhanced expression of
tissue-type plasminogen activator (tPA), urokinase-type plasminogen
activator (uPA), its receptor (uPAR), and plasminogen activator
inhibitors 1 and 2 (PAI-1 and PAI-2), over a several-day holding
period. Immunized mice demonstrated a lower and shorter expression of
these factors over the same period. Expression of these factors at the
mRNA and protein levels may have been due to enzymes and inhibitors
present in inflammatory cells and in resident corneal cells.
conclusions. These results show a correlation between the overexpression of the PA
system in infected naïve mice as part of the inflammatory
response, with eventual ocular destruction. Immunized mice exhibit a
more balanced and shorter expression of the PA system, which may
contribute to the restoration of corneal clarity.
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