Purchase this article with an account.
Sawako Hayashida-Hibino, Hitoshi Watanabe, Kohji Nishida, Motokazu Tsujikawa, Tatsuya Tanaka, Yuichi Hori, Yoshitsugu Saishin, Yasuo Tano; The Effect of TGF-β1 on Differential Gene Expression Profiles in Human Corneal Epithelium Studied by cDNA Expression Array. Invest. Ophthalmol. Vis. Sci. 2001;42(8):1691-1697.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. TGF-βs regulate cell proliferation and differentiation, and they play
important roles in maintenance of corneal epithelium. However, the
precise function of TGF-βs in the corneal epithelium remains unclear.
In this study, cDNA expression array technology was used to demonstrate
the effect of TGF-β1 on the simultaneous expression of a large number
of genes in cultured human corneal epithelial cells (HCECs). The change
in protein level expression of the specific genes influenced by
TGF-β1 was also investigated.
methods. Human cDNA expression array technology was used to study the
simultaneous expression of 1176 specific cellular genes in HCECs
incubated with TGF-β1 (10 ng/ml). Moreover, gene-specific
semiquantitative reverse transcription–polymerase chain reaction
(RT-PCR) was used to confirm the gene expression pattern measured by
the cDNA expression array. Western blot analysis was used to examine
protein expression of the specific genes in the presence or absence of
results. TGF-β1 significantly upregulated the expression of 19 genes and
significantly downregulated ras-related protein, caspase10, andβ
4-integrin in the treated HCECs. The expression of 277 genes
including α3-integrin, PAI-2, transferrin receptor, and cyclin-D1 was
studied. Semiquantitative RT-PCR analysis confirmed the
TGF-β1–mediated changes in expression patterns of these genes.
Furthermore, Western blot analysis revealed that TGF-β1 remarkably
decreased PAI-2, transferrin receptor, and integrin α3, and increased
caspase10 on the protein level.
conclusions. TGF-β1 regulates the expression of specific types of genes in HCECs.
These results strongly suggest that TGF-β1 is critically involved in
the maintenance of the corneal epithelium through the control of a
network of various signal-transduction
This PDF is available to Subscribers Only