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Gülgün Tezel, Lin Ya Li, Raj V. Patil, Martin B. Wax; TNF-α and TNF-α Receptor-1 in the Retina of Normal and Glaucomatous Eyes. Invest. Ophthalmol. Vis. Sci. 2001;42(8):1787-1794.
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purpose. To determine the expression and localization of tumor necrosis factor
(TNF)-α and TNF-α receptor-1 in the retina of normal and
methods. Using immunohistochemistry and in situ hybridization, retinal
expression and localization of TNF-α and TNF-α receptor-1 were
studied in retina sections from 20 eyes of donors with glaucoma, and 20
eyes of age-matched normal donors.
results. According to immunohistochemistry, the intensity of the immunostaining
and the number of labeled cells for TNF-α or its receptor were
greater in retina sections of glaucomatous eyes than in control eyes of
age-matched normal donors. In situ hybridization showed that mRNA
signals for TNF-α or TNF-α receptor-1 were similarly more intense
in glaucomatous eyes than in age-matched control eyes. Both protein and
mRNA of TNF-α or TNF-α receptor-1 were predominantly localized to
the inner retinal layers. Double-immunofluorescence labeling
demonstrated that retinal immunostaining for TNF-α was predominantly
positive in the glial cells, whereas immunostaining for TNF-α
receptor-1 was mainly positive in the retinal ganglion cells.
conclusions. Upregulation of TNF-α and its receptor-1 in glaucomatous retina
suggest that TNF-α–mediated cell death is involved in the
neurodegeneration process of glaucoma.
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