Purchase this article with an account.
Christopher J. Murphy, Carl F. Marfurt, Allison McDermott, Ellison Bentley, George A. Abrams, Ted W. Reid, Sean Campbell; Spontaneous Chronic Corneal Epithelial Defects (SCCED) in Dogs: Clinical Features, Innervation, and Effect of Topical SP, with or without IGF-1. Invest. Ophthalmol. Vis. Sci. 2001;42(10):2252-2261.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To delineate the clinical features and alterations in innervation and
substance P (SP) content in spontaneous chronic corneal epithelial
defects (SCCED) in dogs and to conduct a preliminary investigation
evaluating the efficacy of topical SP, with or without insulin-like
growth factor (IGF)-1, in the treatment of this disorder.
methods. Complete ophthalmic examinations, including Cochet-Bonnet
aesthesiometry, were performed in 45 canine patients that had
spontaneous corneal epithelial defects of at least 3 weeks’ duration
and with no identifiable cause. Eighteen patients had superficial
keratectomies performed, and the corneal nerves were labeled
immunohistochemically with antibodies against protein gene product
(PGP)-9.5, SP, vasoactive intestinal peptide (VIP), and tyrosine
hydroxylase (TH). Relative fiber densities were assessed qualitatively
and quantitatively. Corneal epithelial cell and tear SP contents were
determined in affected and normal dogs by an enzyme immunoassay. A
preliminary open-label treatment trial of topical SP, with and without
IGF-1, was conducted in 21 dogs.
results. The duration of the erosion before admittance into the study was a mean
of 9.22 weeks (range, 3–52). The average patient was middle aged
(mean, 9.25 ± 1.85 years [SD]); no sex predisposition of the
disease was identified. Boxers, golden retrievers, and keeshonds were
overrepresented when compared with the normal hospital population.
Corneal sensation was normal. Marked alterations in corneal innervation
were identified in affected dogs with abnormal increased SP and
calcitonin gene-related peptide (CGRP)–immunoreactive nerve plexuses
identified surrounding the periphery of the epithelial defect. The SP
content of epithelial cells surrounding the defect increased, whereas
the tear SP content remained unchanged. Of the canine patients treated
with SP, with or without IGF-1, 70% to 75% had complete healing of
conclusions. This idiopathic spontaneous corneal disease in dogs shares clinical
features with chronic epithelial defects in humans. The presence of
marked alterations in peptidergic innervation and positive response to
topical therapy with SP suggest that SP plays a critical role in
corneal wound-healing processes.
This PDF is available to Subscribers Only