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Dino D. Klisovic, M. Sue O’Dorisio, Steven E. Katz, Jeffrey W. Sall, Douglas Balster, Thomas M. O’Dorisio, Elson Craig, Martin Lubow; Somatostatin Receptor Gene Expression in Human Ocular Tissues: RT-PCR and Immunohistochemical Study. Invest. Ophthalmol. Vis. Sci. 2001;42(10):2193-2201.
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purpose. Somatostatin (SST) analogues have been used to treat proliferative
diabetic retinopathy, pseudotumor cerebri, thyroid orbitopathy, and
cystoid macular edema. There is a paucity of published data in regards
to cell-specific distribution of SST receptors (SSTR) in normal human
eye tissues. Gene expression for all five known SSTRs in normal human
ciliary body/iris, retina, choroid, and cultured retinal pigment
epithelial (RPE) cells were studied.
methods. mRNA was isolated from human ocular tissues (iris/ciliary body, retina,
and choroid) dissected from eight pairs of normal eyes (9–62 years)
and from RPE cells grown in culture. RT-PCR was done for all five SSTRs
in all analyzed tissues. Immunohistochemistry for SSTR1 and SSTR2 was
performed on eight pairs of normal human eyes (28–74 years) imbedded
results. SSTR1 to 5 genes are expressed in retina, SSTR1 and SSTR2 genes in
cultured RPE cells, and SSTR1, 2, and 4 in ciliary body and choroid.
SSTR1 and SSTR2 immunoreactivity (-ir) was observed on a variety of
cells within all analyzed tissues including cornea, iris, trabecular
meshwork, Schlemm’s canal, ciliary processes, ciliary muscle, retina,
choroid, cultured RPE cells, and optic nerve.
conclusions. SSTR genes are widely expressed in normal human eye tissues, with genes
for SSTR1 and SSTR2 being the most widely expressed. Genes for all
SSTRs are expressed in retina. SSTR1-ir and SSTR2-ir were observed in
all analyzed ocular tissues. Detailed knowledge of SSTRs distribution
and function in the human eye will result in a better understanding of
their role in health and disease.
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